5YZ4

Structure of the PIN domain endonuclease Utp24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979135
Spacegroup name	C 2 2 21
Unit cell lengths	48.740, 73.328, 74.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.591 - 2.135
R-factor	0.2282
Rwork	0.222
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mj7
RMSD bond length	0.008
RMSD bond angle	0.903
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.170
High resolution limit [Å]	2.130	2.130
Rmerge	0.184	1.000
Number of reflections	7270	284
<I/σ(I)>	18.9	1.9
Completeness [%]	94.5	73.6
Redundancy	12.1	7.3
CC(1/2)		0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.2M calcium acetate and 20% polyethylene glycol 3350