5YYM
Crystal structures of E.coli arginyl-trna synthetase (argrs) in complex with substrate Arg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97940 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.727, 244.510, 52.460 |
Unit cell angles | 90.00, 90.69, 90.00 |
Refinement procedure
Resolution | 40.750 - 2.200 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bs2 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.698 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.306 | |
Number of reflections | 57753 | |
<I/σ(I)> | 7.5 | 2.9 |
Completeness [%] | 91.5 | 88.9 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 22% PEG8 000, 0.1 M NaH2PO4/Na2HPO4 pH 6.5 |