5YY0

Crystal structure of the HyhL-HypA complex (form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	95
Detector technology	CCD
Collection date	2016-12-08
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 2 3
Unit cell lengths	132.046, 132.046, 132.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.015 - 3.243
R-factor	0.2918
Rwork	0.290
R-free	0.31370
RMSD bond length	0.003
RMSD bond angle	0.793
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.015	3.360
High resolution limit [Å]	3.243	3.243
Rmerge	0.113	1.622
Number of reflections	12459
<I/σ(I)>	23.6
Completeness [%]	100.0
Redundancy	20.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1M NaCl, 0.1M MgCl2, 12% PEG 3350, 0.1M imidazole-HCl (pH 6.2)