5YXT
Crystal structure of reducing end xylose-releasing exo-oligoxylanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-29 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 |
Unit cell lengths | 54.570, 78.990, 89.148 |
Unit cell angles | 100.34, 95.86, 106.35 |
Refinement procedure
Resolution | 22.350 - 1.880 |
R-factor | 0.2454 |
Rwork | 0.244 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wu4 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.825 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2474: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.350 | 1.940 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.074 | |
Number of reflections | 105939 | |
<I/σ(I)> | 5.2 | |
Completeness [%] | 93.0 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium thiocyanate, PEG 3350 |