5YXH
Structure of Rheb-GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.166, 58.963, 64.345 |
| Unit cell angles | 110.53, 90.03, 108.16 |
Refinement procedure
| Resolution | 59.800 - 2.040 |
| R-factor | 0.24636 |
| Rwork | 0.244 |
| R-free | 0.29862 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xtq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.167 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.800 | 2.090 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 37982 | |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 91.5 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 25% w/v PEG 1500 |
