5YXE
Crystal Structure Analysis of feline serum albumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.908, 49.145, 123.869 |
| Unit cell angles | 90.00, 110.17, 90.00 |
Refinement procedure
| Resolution | 46.795 - 3.402 |
| R-factor | 0.286492746435 |
| Rwork | 0.286 |
| R-free | 0.29674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lu8 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.493 |
| Data reduction software | XDS (May 1, 2016) |
| Data scaling software | XDS (May 1, 2016) |
| Phasing software | MOLREP (11.2.08) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 3.610 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 8375 | 2486 |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.973 | 0.617 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 293 | 47mg/mL FSA in 10mM Na Phosphate pH7.4, 35% PEG3350, 0.2M Na malonate pH4.8 |
