5YWU
Crystal structure of TREX1 in complex with a inosine contained dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 133.180, 133.180, 76.282 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.780 - 3.400 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mxm |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.582 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.075 | 0.486 |
| Number of reflections | 9877 | |
| <I/σ(I)> | 24.6 | 2.9 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 12.2 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 0.2M POTASSIUM CITRATE TRIBASIC MONOHYDRATE, PH 8.3, 20%(W/V) POLYETHYLENE GLYCOL 3,350, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
