5YWR

Crystal Structure of RING E3 ligase ZNRF1 in complex with Ube2N (Ubc13)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979470
Spacegroup name	P 21 21 21
Unit cell lengths	47.430, 59.548, 94.792
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.417 - 1.470
R-factor	0.1659
Rwork	0.165
R-free	0.18420
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.683
Data reduction software	XDS
Data scaling software	pointless
Phasing software	SHELXD
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.430	1.500
High resolution limit [Å]	1.470	1.470
Rmerge	0.055	0.872
Rpim	0.031	0.587
Number of reflections	46073
<I/σ(I)>	20.6
Completeness [%]	99.2
Redundancy	7.7
CC(1/2)		0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	0.2M Magnesium fromate dihydrate, 20% PEG 3350 (w/v)