5YWL

SsCR_L211H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	47.755, 53.150, 140.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.432 - 2.098
R-factor	0.2088
Rwork	0.206
R-free	0.26540
RMSD bond length	0.008
RMSD bond angle	1.047
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.432	2.140
High resolution limit [Å]	2.098	2.100
Number of reflections	21187
<I/σ(I)>	20.9
Completeness [%]	97.9
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M lithium sulfate monohydrate, 0.1M Tris hydrochloride (pH 8.5), 30% (w/v) PEG 4000