5YVG
Crystal structure of Karyopherin beta2 in complex with FUS(full length)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2012-11-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.219, 145.015, 108.681 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 41.330 - 4.050 |
| R-factor | 0.3653 |
| Rwork | 0.251 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z5k |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.689 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | phenix.refine (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.070 |
| High resolution limit [Å] | 4.050 | 4.050 |
| Rpim | 0.056 | 0.472 |
| Number of reflections | 27683 | 1301 |
| <I/σ(I)> | 10.9 | 1.3 |
| Completeness [%] | 99.2 | 95.1 |
| Redundancy | 4.1 | |
| CC(1/2) | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.8M Succinic acid pH7.0 |
