5YUY
DNA polymerase IV - DNA ternary complex 9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-18 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.460, 57.050, 110.170 |
| Unit cell angles | 90.00, 95.03, 90.00 |
Refinement procedure
| Resolution | 44.542 - 1.740 |
| R-factor | 0.1895 |
| Rwork | 0.188 |
| R-free | 0.21530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ir1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.870 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.060 | 0.587 |
| Number of reflections | 110068 | 15993 |
| <I/σ(I)> | 12.1 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.996 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 15% MPD , pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
