5YT8

Crystal structure of H5 hemagglutinin with G228S Q226L mutants from A/chicken/Taiwan/0502/2012

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	111.170, 111.170, 313.782
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.676 - 2.765
R-factor	0.2022
Rwork	0.199
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.095
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.870
High resolution limit [Å]	2.765	2.765
Number of reflections	19535	1927
<I/σ(I)>	20.4
Completeness [%]	99.8	100
Redundancy	4.8	4.8
CC(1/2)		0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M tris, 0.2M MgCl, 14% PEG8000