5YSX

Structure of P domain of GII.2 Noroviruses

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	113
Detector technology	CCD
Collection date	2017-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 32 2 1
Unit cell lengths	96.534, 96.534, 64.319
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.361 - 1.202
R-factor	0.1547
Rwork	0.154
R-free	0.16710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rpb
RMSD bond length	0.009
RMSD bond angle	1.079
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.240
High resolution limit [Å]	1.200	1.200
Number of reflections	99924
<I/σ(I)>	29.5
Completeness [%]	99.7
Redundancy	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	291	0.1 M Sodium Acetate trihydrate pH 4.6, 8% w/v Polyethylene Glycol 4000