5YSU
Plumbagin in complex with CRM1-RanM189D-RanBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 105.063, 105.063, 306.534 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.740 - 2.300 |
| R-factor | 0.2224 |
| Rwork | 0.221 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hat |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.454 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.155 | 0.070 | |
| Rmeas | 0.156 | 0.073 | |
| Rpim | 0.058 | 0.021 | 0.602 |
| Total number of observations | 911944 | ||
| Number of reflections | 77361 | 4257 | 3818 |
| <I/σ(I)> | 2.6 | ||
| Completeness [%] | 100.0 | 99.9 | 99.9 |
| Redundancy | 11.8 | 12.1 | 11.5 |
| CC(1/2) | 0.998 | 0.319 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 18% PEG 3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH 6.6 |
