5YSI
SdeA mART-C domain EE/AA NCA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2017-05-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 37.867, 75.698, 84.682 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.555 - 1.546 |
R-factor | 0.208 |
Rwork | 0.204 |
R-free | 0.24580 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.091 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.555 | 1.602 |
High resolution limit [Å] | 1.546 | 1.546 |
Rmerge | 0.542 | |
Number of reflections | 36284 | 1754 |
<I/σ(I)> | 32.37 | |
Completeness [%] | 99.6 | 97.33 |
Redundancy | 6.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG400, Tris |