5YSD
Crystal structure of beta-1,2-glucooligosaccharide binding protein in complex with sophorotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.082, 125.561, 91.780 |
| Unit cell angles | 90.00, 101.61, 90.00 |
Refinement procedure
| Resolution | 44.950 - 2.100 |
| R-factor | 0.17139 |
| Rwork | 0.169 |
| R-free | 0.20732 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yse |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.851 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.116 | 0.639 |
| Rpim | 0.071 | 0.389 |
| Number of reflections | 44909 | 2234 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 96.5 | 96.5 |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 0.697 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.15M MES-NaOH, pH 5.5, 50% (v/v) MPD |
