5YS6

Structure of the ectodomain of pseudorabies virus glycoproten B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	H 3
Unit cell lengths	100.288, 100.288, 272.918
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.569 - 3.100
R-factor	0.229
Rwork	0.227
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gum
RMSD bond length	0.006
RMSD bond angle	0.982
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Number of reflections	18750
<I/σ(I)>	15.779
Completeness [%]	99.9
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	34% PEG 200, 0.1 M citric acid