5YS4
Crystal structure of retroviral protease-like domain of Ddi1 from Leishmania major
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 |
Unit cell lengths | 43.790, 56.780, 91.400 |
Unit cell angles | 76.56, 84.48, 89.99 |
Refinement procedure
Resolution | 26.340 - 2.300 |
R-factor | 0.238 |
Rwork | 0.236 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i1a |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1-2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.340 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.145 | 0.800 |
Number of reflections | 36968 | 3613 |
<I/σ(I)> | 4.9 | 1.8 |
Completeness [%] | 97.6 | 96.8 |
Redundancy | 2.7 | 2.7 |
CC(1/2) | 0.985 | 0.707 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.5 | 293 | 0.1M sodium malonate, 12 % polyethylene glycol 3350 |