5YRG

PPL3A-isomaltose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-29
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	45.950, 50.120, 118.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.842 - 1.500
R-factor	0.1789
Rwork	0.177
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yrk
RMSD bond length	0.010
RMSD bond angle	1.145
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.020	0.113
Number of reflections	44443	6432
<I/σ(I)>	17.6	5.1
Completeness [%]	99.7	99.7
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M ammonium sulfate, 25%(w/v) PEG 3350, 0.1M Tris buffer pH 5.5