5YR7

Human methionine aminopeptidase type 1b (F309L mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-03-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	47.414, 77.193, 48.220
Unit cell angles	90.00, 92.03, 90.00

Refinement procedure

Resolution	23.690 - 2.060
R-factor	0.1991
Rwork	0.196
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3k
RMSD bond length	0.015
RMSD bond angle	1.699
Data reduction software	DENZO (2.3.1)
Data scaling software	SCALEPACK (2.2.0)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.130
High resolution limit [Å]	2.060	4.430	2.060
Rmerge	0.068	0.040	0.450
Rmeas	0.088	0.051	0.586
Rpim	0.055	0.032	0.371
Number of reflections	17388	1806	1684
<I/σ(I)>	12.8
Completeness [%]	79.9	81.1	78.1
Redundancy	2	2.1	1.9
CC(1/2)		0.994	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	0.1M Bistris pH-6.2, 19% PEG 3350, 5% Glycerol