5YR2
Structure of cpGFP66BPA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2016-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.640, 63.709, 69.159 |
| Unit cell angles | 90.00, 101.09, 90.00 |
Refinement procedure
| Resolution | 39.183 - 1.799 |
| R-factor | 0.1703 |
| Rwork | 0.168 |
| R-free | 0.21460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gfl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.148 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.799 | 1.799 |
| Number of reflections | 39464 | 3883 |
| <I/σ(I)> | 21.17 | |
| Completeness [%] | 98.5 | 97.5 |
| Redundancy | 5.6 | 5.6 |
| CC(1/2) | 0.996 | 0.912 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289.15 | PEG 3350 25%, 0.2M MgCl2,0.1M Bis-Tris pH 5.5 |
