5YQX
Crystal Structure Analysis of the BRD4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-04-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97776 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.410, 47.280, 60.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.280 - 1.820 |
R-factor | 0.1639 |
Rwork | 0.161 |
R-free | 0.21880 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.878 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.5.17) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.610 | 1.860 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.058 | 0.230 |
Rmeas | 0.061 | 0.240 |
Rpim | 0.018 | 0.068 |
Number of reflections | 11689 | 654 |
<I/σ(I)> | 25.2 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 11.4 | 12.1 |
CC(1/2) | 0.999 | 0.986 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES pH 7.5, 20% PEG 3350, 10% EtGhly, 0.2M NaCl |