5YQQ
Crystal structure of a domain-swapped dimer of the second StARkin domain of Lam2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.862, 52.124, 53.021 |
| Unit cell angles | 107.81, 90.17, 105.05 |
Refinement procedure
| Resolution | 24.017 - 1.900 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.24210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yqj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.862 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.045 | 0.279 |
| Number of reflections | 25976 | 1273 |
| <I/σ(I)> | 32.3 | 5.5 |
| Completeness [%] | 97.4 | 96.6 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1 M Tris-HCl pH 8.0, 30% PEG 8000 |
