5YQP

Crystal structure of the second StARkin domain of Lam4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-29
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97950
Spacegroup name	I 2 2 2
Unit cell lengths	68.337, 89.074, 111.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.232 - 1.700
R-factor	0.2043
Rwork	0.202
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yqj
RMSD bond length	0.007
RMSD bond angle	0.923
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.071	0.383
Number of reflections	37918	1880
<I/σ(I)>	51.7	8.2
Completeness [%]	99.6	100
Redundancy	8.8	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	295	0.1M Bicine-NaOH pH 9.0, 30% PEG1500, 0.1M MgCl2