5YQI
Crystal structure of the first StARkin domain of Lam2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.433, 59.505, 84.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.973 - 1.600 |
R-factor | 0.2078 |
Rwork | 0.206 |
R-free | 0.24630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yqj |
RMSD bond length | 0.006 |
RMSD bond angle | 0.746 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.060 | 0.427 |
Number of reflections | 22632 | 1117 |
<I/σ(I)> | 45.5 | 5.3 |
Completeness [%] | 98.6 | 100 |
Redundancy | 5.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1M MES-NaOH pH 6.0, 30% PEG3350, 0.1M KNO3 |