5YQG

The structure of 14-3-3 and pNumb peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	59.260, 74.626, 134.136
Unit cell angles	90.00, 90.07, 90.00

Refinement procedure

Resolution	49.883 - 2.100
R-factor	0.1858
Rwork	0.184
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hkc
RMSD bond length	0.007
RMSD bond angle	0.803
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.086
Number of reflections	66596
<I/σ(I)>	2.1
Completeness [%]	98.2
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	290.15	tri-Sodium Citrate dihydrate, isopropanol, PEG 4000