5YPS
The structural basis of histone chaperoneVps75
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-04-05 |
| Detector | OXFORD SAPPHIRE CCD |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 141.672, 76.404, 130.448 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.260 - 2.097 |
| R-factor | 0.1881 |
| Rwork | 0.186 |
| R-free | 0.23580 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.085 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.260 |
| High resolution limit [Å] | 2.090 |
| Number of reflections | 83282 |
| <I/σ(I)> | 23.8 |
| Completeness [%] | 100.0 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | Calcium chloride dihydrate, HEPES sodium, PEG 400 |
