5YPH
p62/SQSTM1 ZZ domain with Ile-peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-02-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.658, 33.381, 35.019 |
| Unit cell angles | 90.00, 103.61, 90.00 |
Refinement procedure
| Resolution | 17.909 - 1.629 |
| R-factor | 0.1511 |
| Rwork | 0.147 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yp7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.305 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 17.910 |
| High resolution limit [Å] | 1.629 |
| Number of reflections | 9011 |
| <I/σ(I)> | 34.7 |
| Completeness [%] | 94.0 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | sodium phosphate, potassium phosphate, MgCl2 |
