5YOW
The post-fusion structure of the Heartland virus Gc glycoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 3 |
Unit cell lengths | 117.875, 117.875, 117.875 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.292 - 2.100 |
R-factor | 0.1853 |
Rwork | 0.182 |
R-free | 0.23390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g47 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.246 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.032 | 0.495 |
Number of reflections | 32160 | 3200 |
<I/σ(I)> | 23.3 | 1.65 |
Completeness [%] | 100.0 | 99 |
Redundancy | 12.8 | 6.8 |
CC(1/2) | 0.538 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 6% (v/v) 2-propanol, 0.1M sodium acetate trihydrate, pH 4.5, 26%(v/v) polyethylene glycol monomethyl ether 550 |