5YOB
Crystal Structure of flavodoxin without engineered disulfide bond
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-06 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.232, 56.246, 41.058 |
| Unit cell angles | 90.00, 100.84, 90.00 |
Refinement procedure
| Resolution | 31.656 - 1.142 |
| R-factor | 0.1668 |
| Rwork | 0.166 |
| R-free | 0.17740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j8q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.088 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1-2155-000) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.656 |
| High resolution limit [Å] | 1.142 |
| Number of reflections | 38709 |
| <I/σ(I)> | 32.21 |
| Completeness [%] | 75.0 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris-HCL 0.1M Ammonium Sulfate 3.2M |
