5YNF

Crystal structure of MERS-CoV nsp16/nsp10 complex bound to m7GpppA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-03
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9778
Spacegroup name	P 21 2 21
Unit cell lengths	66.658, 70.492, 118.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.433 - 1.791
R-factor	0.1808
Rwork	0.179
R-free	0.21290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r24
RMSD bond length	0.016
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.450
High resolution limit [Å]	1.790	1.790
Rmerge	0.058
Rmeas	0.061
Rpim	0.018
Number of reflections	105592	5208
<I/σ(I)>	22	3.3
Completeness [%]	99.3	99.2
Redundancy	11	10.8
CC(1/2)	1.000	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	10% PEG 5000 ME, 5% Tascimate, pH7.0