5YMX
Myxococcus xanthus MglA in GDP bound conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.340, 68.610, 89.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.924 - 1.350 |
R-factor | 0.152 |
Rwork | 0.150 |
R-free | 0.17870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t12 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.878 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.030 | 1.420 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.055 | 0.734 |
Rmeas | 0.060 | 0.791 |
Rpim | 0.022 | 0.294 |
Number of reflections | 91269 | 13188 |
<I/σ(I)> | 14.6 | 2.6 |
Completeness [%] | 99.7 | 100 |
Redundancy | 7.2 | 7.2 |
CC(1/2) | 0.999 | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30% 2-Methyl 2,4-pentane diol (MPD), 10% PEG 4000, 100mM Imidazole, pH 8.0 |