5YMA

Crystal structure of ribosome assembly factor Efg1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-22
Detector	Nonius Kappa CCD
Wavelength(s)	0.9792
Spacegroup name	P 62
Unit cell lengths	99.122, 99.122, 90.179
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.755 - 3.295
R-factor	0.2035
Rwork	0.198
R-free	0.25170
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.360
High resolution limit [Å]	3.295	3.300
Rmerge	0.362	1.000
Number of reflections	7701	385
<I/σ(I)>	19.3
Completeness [%]	99.0	100
Redundancy	22	20.1
CC(1/2)		0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	2.4M sodium malonate (pH 7.0)