5YM0

The crystal structure of DHAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.96422
Spacegroup name	P 42 21 2
Unit cell lengths	135.472, 135.472, 67.963
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.900 - 1.842
R-factor	0.171
Rwork	0.170
R-free	0.19740
RMSD bond length	0.009
RMSD bond angle	0.962
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.840
Number of reflections	55067
<I/σ(I)>	22.6
Completeness [%]	99.8
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	0.1M sodium acetate pH 5.5, 1.2 M ammonium sulfate