5YL1

T=1 subviral particle of Penaeus vannamei nodavirus capsid protein deletion mutant (delta 1-37 & 251-368)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-09
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	196.981, 200.319, 419.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.620 - 3.120
R-factor	0.21959
Rwork	0.220
R-free	0.22071
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.696
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.620	3.230
High resolution limit [Å]	3.120	3.120
Rmeas	0.244	0.963
Rpim	0.108	0.428
Number of reflections	292229
<I/σ(I)>	7.1
Completeness [%]	99.7	99.8
Redundancy	5	5
CC(1/2)		0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Tris 8.0, 20%(w/v) Poly(acrylic acid sodium salt) 5100