5YKC
crystal structure of H5 hemagglutinin from A/chicken/Taiwan/0502/2012
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.424, 220.103, 186.508 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.036 - 2.823 |
| R-factor | 0.2011 |
| Rwork | 0.197 |
| R-free | 0.27250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e2y |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.266 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.040 | 2.920 |
| High resolution limit [Å] | 2.823 | 2.820 |
| Rmerge | 0.074 | 0.685 |
| Number of reflections | 46855 | |
| <I/σ(I)> | 14.14 | |
| Completeness [%] | 85.4 | 99.2 |
| Redundancy | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M tris, 0.2M MgCl, 14% PEG8000 |
