5YJZ
The native crystal structure of Rv3197 from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00849 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 187.290, 187.290, 67.609 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.390 - 2.160 |
| R-factor | 0.1781 |
| Rwork | 0.176 |
| R-free | 0.20800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.035 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.062 | 0.270 |
| Number of reflections | 37838 | 1817 |
| <I/σ(I)> | 45.8 | 8.2 |
| Completeness [%] | 99.8 | 97.7 |
| Redundancy | 14.3 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 2.0M lithium sulfate, 0.1 M Tris (pH 8.5), 2% (w/v) PEG 400 |
