5YJ3

Crystal structure of TZAP and telomeric DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978
Spacegroup name	P 43 21 2
Unit cell lengths	56.713, 56.713, 226.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.279 - 2.845
R-factor	0.2281
Rwork	0.226
R-free	0.26780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w0t
RMSD bond length	0.004
RMSD bond angle	0.590
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.920
High resolution limit [Å]	2.845	7.020	2.850
Rmerge	0.071	0.041	0.983
Rmeas	0.073	0.042	1.007
Rpim	0.015	0.009	0.214
Number of reflections	9428	747	582
<I/σ(I)>	4.8
Completeness [%]	100.0	99.9	100
Redundancy	23.7	19.3	21.2
CC(1/2)		1.000	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2 M Imidazole-Malate pH 8.5, 20% PEG 4K