5YJ0
Mouse Cereblon thalidomide binding domain complexed with S-form thalidomide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-18 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.28268 |
| Spacegroup name | H 3 |
| Unit cell lengths | 201.897, 201.897, 123.614 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.150 - 1.800 |
| R-factor | 0.18972 |
| Rwork | 0.189 |
| R-free | 0.20425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wx2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.665 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.081 | 0.500 |
| Number of reflections | 173436 | 17239 |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1.0M ammonium sulfate 0.1M sodium acetate (pH5.0) |
