5YIV

Caulobacter crescentus GcrA DNA-binding domain(DBD) in complex with methylated dsDNA(crystal form 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 2
Unit cell lengths	124.017, 131.704, 77.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.030 - 2.914
R-factor	0.2028
Rwork	0.201
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yiu
RMSD bond length	0.004
RMSD bond angle	0.728
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.110
Rmeas	0.120
Rpim	0.050
Number of reflections	13413	1010
<I/σ(I)>	12.4	1.1
Completeness [%]	94.3	92.2
Redundancy	4.8
CC(1/2)		0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	30% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, 0.2M Ammonium surface