5YI6
CRISPR associated protein Cas6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 200.835, 85.255, 100.065 |
| Unit cell angles | 90.00, 118.47, 90.00 |
Refinement procedure
| Resolution | 29.087 - 1.852 |
| R-factor | 0.1673 |
| Rwork | 0.167 |
| R-free | 0.19450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i4h |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.025 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.087 | 1.919 |
| High resolution limit [Å] | 1.852 | 1.852 |
| Rmerge | 0.501 | |
| Rmeas | 0.301 | |
| Rpim | 0.586 | |
| Number of reflections | 125357 | 12437 |
| <I/σ(I)> | 17.28 | 2.039 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100mM HEPES sodium salt pH7.5, 600mM NaH2PO4, 600mM KH2PO4 |
