5YHM
Crystal structure of dehydroquinate dehydratase with tris induced oligomerisation at 1.907 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.904, 155.606, 155.912 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.700 - 1.910 |
| R-factor | 0.20044 |
| Rwork | 0.200 |
| R-free | 0.24779 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b6p |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.687 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.700 | 1.956 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rpim | 0.134 | |
| Number of reflections | 139447 | 1715 |
| <I/σ(I)> | 5.96 | |
| Completeness [%] | 89.8 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.5M Ammonium sulphate, 0.1M tris, 12% Glycerol |
