5YHI
Crystal structure of YiiM from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.162, 84.837, 107.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.850 |
R-factor | 0.20345 |
Rwork | 0.201 |
R-free | 0.24227 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o65 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.283 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.122 | 0.430 |
Number of reflections | 12483 | 590 |
<I/σ(I)> | 14.7 | 2.3 |
Completeness [%] | 99.7 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.1M ammonium dihydrogen phosphate, 0.08M CHES, pH 9.5, 20% glycerol |