5YHF
Crystal structure of SecDF in Super-membrane-facing form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-14 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 55.190, 65.640, 79.980 |
Unit cell angles | 75.11, 75.23, 80.83 |
Refinement procedure
Resolution | 48.864 - 2.800 |
R-factor | 0.2067 |
Rwork | 0.203 |
R-free | 0.24640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3aqp |
RMSD bond length | 0.002 |
RMSD bond angle | 0.455 |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.864 | 48.864 | 2.970 |
High resolution limit [Å] | 2.800 | 8.370 | 2.800 |
Rmerge | 0.066 | 0.140 | 0.905 |
Rmeas | 0.671 | 0.142 | 9.251 |
Total number of observations | 590974 | ||
Number of reflections | 25694 | 956 | 4166 |
<I/σ(I)> | 8.23 | 27.48 | 1.81 |
Completeness [%] | 99.8 | 99.4 | 100 |
Redundancy | 23 | 25.209 | 22.61 |
CC(1/2) | 0.987 | 0.995 | 0.789 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 8.5 | 298 | 50% PEG 400, 100 mM Tris-HCl pH 8.5, 100 mM KCl |