5YH5
The crystal structure of Staphylococcus aureus CntA in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2017-01-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 61 |
| Unit cell lengths | 149.395, 149.395, 53.635 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.500 - 2.900 |
| R-factor | 0.19369 |
| Rwork | 0.190 |
| R-free | 0.26367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.046 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 15367 |
| <I/σ(I)> | 16.7 |
| Completeness [%] | 100.0 |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M MES monohydrate pH 5.5, 25% (v/v) Polyethylene glycol 400 |
