5YH4
Miraculin-like protein from Vitis vinifera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.040, 59.600, 74.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.930 - 1.300 |
| R-factor | 0.17492 |
| Rwork | 0.174 |
| R-free | 0.18824 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zc8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.355 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.930 |
| High resolution limit [Å] | 1.300 |
| Number of reflections | 50318 |
| <I/σ(I)> | 11.9 |
| Completeness [%] | 100.0 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1 M MES buffer (pH 6.5), 12% PEG 20000 |
