5YH0
The structure of DrFam20C1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.925, 135.988, 219.971 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 47.843 - 3.450 |
| R-factor | 0.2259 |
| Rwork | 0.225 |
| R-free | 0.27850 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.003 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.450 |
| Number of reflections | 88916 |
| <I/σ(I)> | 8 |
| Completeness [%] | 99.5 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 0.2 M Ammonium citrate tribasic pH 7.0, 0.1 M Imidazole pH 7.0, 18% w/v Polyethylene glycol monomethyl ether 2,000 |
