5YGS
Human TNFRSF25 death domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-07-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.032920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.690, 153.550, 98.240 |
| Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
| Resolution | 98.250 - 2.691 |
| Rwork | 0.190 |
| R-free | 0.21250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1anf |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.12-2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.250 | 2.793 |
| High resolution limit [Å] | 2.691 | 2.696 |
| Rmerge | 1.120 | |
| Number of reflections | 59937 | |
| <I/σ(I)> | 18.53 | |
| Completeness [%] | 99.6 | 96.56 |
| Redundancy | 7.335 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2.6 M ammonium sulfate, sodium citrate 5.6, 10% glycerol |
