5YGR
Crystal structure of PLP bound Diaminopropionate ammonia lyase from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.956 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.890, 128.440, 138.100 |
| Unit cell angles | 90.00, 110.28, 90.00 |
Refinement procedure
| Resolution | 77.000 - 2.500 |
| R-factor | 0.23953 |
| Rwork | 0.237 |
| R-free | 0.29919 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d9g |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.457 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.160 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.130 | 0.640 |
| Rpim | 0.070 | 0.330 |
| Number of reflections | 58141 | 8417 |
| <I/σ(I)> | 7.5 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.6 | 4.5 |
| CC(1/2) | 0.980 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.6 | 298 | 20%(w/v) PEG4000, 20%(v/v) 2-propanol, 100mM sodium citrate pH 5.6, temperature 298K |
