5YGI

Crystal structure of human FPPS in complex with an inhibitor THZ93

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-05
Detector	Nonius Kappa CCD
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	111.743, 111.743, 66.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.470 - 2.177
R-factor	0.227
Rwork	0.225
R-free	0.27190
RMSD bond length	0.007
RMSD bond angle	0.830
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	24.470
High resolution limit [Å]	2.177
Number of reflections	22270
<I/σ(I)>	49.2
Completeness [%]	98.9
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.2M Na/K phosphate, 25% glycerol, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K